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i400 USER MANUAL

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This User Manual provides instructions for the following products:

MODEL P.N. HW REV. SW REV.
i400 Intelligent Laboratory Pump FL-i400-LAB-01-01-00-24V GEN 2.0 VER 1.3.x

Document Information
Copyrights & Trademarks

The information provided in this literature was reviewed for accuracy at the time of publication and is believed to be correct, however LAB DYNAMICS, Inc. accepts no liability for any errors it contains and reserves the right to alter specifications without notice. For current information contact LAB DYNAMICS directly. It is the users responsibility to ensure product suitability for use within their application.

Modbus® is a registered trademark of Modicon, Incorporated. All trademarks are the property of their respective owners.

© 2022 LAB DYNAMICS, INC., ALL RIGHTS RESERVED. FLUIDLINK®, LABDYNAMICS®, AND THE RESPECTIVE LOGOS ARE TRADEMARKS OF LAB DYNAMICS, INC.

Immediate Precautions

WARNING: THESE PRODUCTS ARE NOT DESIGNED FOR USE IN, AND SHOULD NOT BE USED FOR PATIENT-CONNECTED APPLICATIONS.

WARNING: KEEP FINGERS AWAY FROM ROTOR WHILE PUMP IS IN OPERATION. STOP PUMP BEFORE LOADING OR UNLOADING TUBING.


Manufacturing Standards

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Electromagnetic Compatibility

This device has been designed to comply with the standards and regulations set down by the European EMC Directive 2014/30/EU using BS EN 61326-1: 2013.

Safety

This device has been designed to comply with the standards and regulations set down by the European Low Voltage Directive 2014/35/EU using BS EN 61010-1: 2010, and Machinery Directive 2006/42/EC using BS EN ISO 12100: 2010.

Quality

This instrument has been manufactured under the following quality standard: ISO 9001:2008 (Certification Pending).

Note: The standards referred to in the design and construction of these products are those prevailing at the time of product launch. As the standards are altered from time to time, we reserve the right to include design modifications that are deemed necessary to comply with the new or revised regulations.

Disposal
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As per directive 2012/19/EU, please observe the applicable local or national regulations concerning the disposal of waste electrical and electronic equipment.


Introduction

The FLUIDLINK® i400 Intelligent Laboratory Pump is a compact, feature-rich peristaltic pump with unmatched ease-of-use and built-in automation connectivity. This User Manual will guide you through the proper setup and operation of the i400 pump and includes important safety information.

Who should read this Manual?
Intended Audience

The User Manual is written for use by Novice Users (who require only the basic operational knowledge) as well as more Advanced Users, including Automation Engineers. The manual is formatted as a "Quick Start" guide, which offers deeper Advanced User topics where applicable. The Quick Start format is meant to encourage reading all necessary topics before operating the pump.

Before You Get Started

Please observe the following:

WHAT TO DO NEXT:

  • Continue scrolling to BASICS OVERVIEW for the essentials.
  • Continue scrolling to APPLICATIONS for Use Cases.
  • Feel free to ask for assistance:

Application Solutions

i400 Use Cases:
LAB-SCALE CELL CULTURE DEVELOPMENT
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Scientists appreciate the FLUIDLINK® i400 pump for its flexibility and accuracy - Operators appreciate its simplicity and intuitive user interface.

WHAT TO DO NEXT:


Basics Overview

The i400 Pump is a highly accurate and easy-to-use laboratory peristaltic pump. Although the touch-screen interface of the pump is highly intuitive, we strongly encourage new users to take a few moments to read this manual. The next section will introduce you to the touch-screen User Interface, followed by a section describing the Physical Connections of the pump.

Touring the User Interface:

The OVERVIEW screen is the initial operational screen you'll encounter, and presents the most frequently used information in a single consolidated view. Take a moment to get familiar with the features found on this screen and features that are common to many other screens.

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    • Click through a quick tour of the User Interface...
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    Figure 1.1: OVERVIEW screen with all indicators shown.

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    • Each operational screen has a consistent grouping of functions at the top and bottom of the display, which appear on all operational screens.
    • Along the top of the screen you'll find:
    • PUMP NAME: helps distinguish one pump from another.
    • SYSTEM SETTINGS: reveals a menu to access system setup options.
    • ROTATION DIRECTION: used to both display and change rotation direction.

    Figure 1.2: OVERVIEW screen showing screen-top indicators.

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    • Along the bottom of the screen you'll find:
    • MODE: will step you from one operational screen to another, eventually returning back to the OVERVIEW screen.
    • RUN/STOP: starts and stops the pump head rotation when operating in MANUAL mode.
    • STATUS: opens a screen to view working status, and address any ALARMS.
    • TASKS: reveals a menu for performing most common system functions.

    Figure 1.3: OVERVIEW screen showing screen-bottom indicators.

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    • The active area of the screen contains:
    • SCREEN NAME: the name of the currently selected operational screen.
    • SYSTEM TIME: the current system time, always shown in 24-hour format.
    • RPM: the current RPMs of the pump head while in motion.
    • mL/min: the current mL/min flow rate of the pump head while in motion.

    Figure 1.4: OVERVIEW screen showing SCREEN NAME, SYSTEM TIME, RPM, and mL/min.

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    • The active area of the screen contains: (cont.)
    • FLUIDLINK.IO: indicates if the REMOTE feature is configured, and when it's actively connected.
    • INDUSTRIAL NETWORK INDICATOR: indicates data flowing from and to a client industrial controller.
    • TUBING: displays the name or description of the currently loaded tubing.

    Figure 1.5: OVERVIEW screen showing FLUIDLINK.IO (REMOTE) & Industrial Network Indicator, and TUBING description.

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    • The active area of the screen contains: (cont.)
    • RUN STATUS: describes the state of the currently running process, or the staged process waiting to be triggered.

    Figure 1.6: OVERVIEW screen showing RUN STATUS indicator.

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    • The active area of the screen contains: (cont.)
    • ACTION SEQUENCE: group of indicators showing the loaded ACTION SEQUENCE ("A-SEQ"), along with a quick-edit button. A-SEQ's are a powerful yet easy-to-use feature exclusive to the i400 pump.



    • Continue scrolling down the page to click through a quick tour of the Physical Connections of the pump...

    Figure 1.7: OVERVIEW screen showing ACTION SEQUENCE indicator group.

Touring the Physical Connections

All signal and electrical connections to the i400 are located on the BACK PANEL. The i400 has an ingress protection rating of IP33 when all connections are secured by their attached caps or terminated with the appropriate connectors.

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    • Click through a quick tour of the Physical Connections...
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    Figure 2.1: BACK PANEL connections.

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    • There are (5) connections on the BACK PANEL:
    • MODBUS / RS-485: 3-wire connection to RS-485 serial bus (RTU).
    • INDUSTRIAL NETWORK: RJ45 connection to Modbus TCP/IP, & EtherNet/IP.
    • MAINTENANCE: RJ45 connection for FLUIDLINK.IO (Remote Control) and synchronizing system time.
    • WEIGHT SCALE: USB (FTDI) RS-232 connection to serial connector. Also used to attach a USB drive to perform Software Updates.
    • POWER: 24V DC input.

    Figure 2.2: BACK PANEL connections (cont.).

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    • The following cables and connectors are packaged with the i400:
    • i400 Scale Serial Cable
      Part #: FL-i400-SIG-1000-NA-00-001
    • i400 RJ45 Industrial Network Cable
      Part #: FL-i400-SIG-1825-NA-00-002
    • i400 DC Power Field Connector
      Part #: FL-i400-PWR-NAA-US-00-002
    • i400 Modbus (C3) Field Connector
      Part #: FL-i400-SIG-NA00-NA-00-003
    • i400 AC-DC Power Converter
      Part #: FL-i400-PWR-NAA-US-00-001

    Figure 2.3: Provided connectors.

WARNING: NEVER SPRAY-DOWN (WASH-DOWN) THE PUMP UNLESS ALL CONNECTORS OR WATER-RESISTANT CAPS ARE SECURELY IN PLACE.

WHAT TO DO NEXT:


Startup

Placing the i400 Pump into service only takes a few minutes, after which all your settings, calibration data, and preferences are permanently stored, and are automatically restored on subsequent reboots. The next set of instructions will step you through the STARTUP and SHUTDOWN process and configuring some of the basic settings.

Powering-up the i400 for the first time:

STARTUP

To power-up the i400 connect the i400 AC-DC Power Converter to the POWER input on the BACK PANEL, then connect the converter to an appropriate AC power source. The i400 was intentionally designed without a power ON/OFF button, in order to prevent accidental interruptions.


SET YOUR LOCAL LANGUAGE

When the i400 is first powered-up a series of STARTUP SCREENS are displayed. If you don't press any options during STARTUP then the i400 will continue to load the FLUIDLINK/OS and display the default OVERVIEW screen.

During STARTUP you're presented the option to change the local language (by pressing the Globe Icon on the initial screen). Your language choice will be saved and can be changed in the future by REBOOTING the system.

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Figure 3.1: Sequence of STARTUP SCREENS.


SHUTDOWN

From the SYSTEM SETTINGS menu (A), select SHUTDOWN... (B), then select SHUTDOWN (C). When the REMOVE POWER screen appears disconnect power to the pump (D).

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Figure 3.2: Sequence of SHUTDOWN SCREENS.

WHAT TO DO NEXT:


Attach Scale

Connect a weight scale to enable a multitude of performance enhancing and time saving features unique to the FLUIDLINK line of products.

Connecting a Weight Scale:

CONNECT SCALE CABLE

Connect the i400 Scale Serial Cable from the WEIGHT SCALE port on the pump to the DB9 serial communications port of your scale. The weight scale must support MT-SICS Command Protocol and be equipped with an RS-232 (DB9) communications interface.

NOTE: "SCALE TASKS" and scale operation details are covered in a later chapter.


SCALE COMMUNICATION SETTINGS

  • Baud Rate: 9600
  • Data Bits: 8
  • Stop Bits: 1
  • Parity: none


SCALE SETUP

From the SYSTEM SETTINGS menu (A), select SCALE SETUP (B), followed by SCALE SETUP (C). Follow the system prompts to complete setup process.

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Figure 4.1: Settings for attaching a weight scale input.

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Animation 4.1: Running SCALE SETUP process.

Note: "SCALE TASKS" and scale operation details are explained in a later section.

WHAT TO DO NEXT:


System Time

The i400 has many features that rely on accurate SYSTEM TIME settings. The next set of instructions will step you through setting the system clock manually, or using a network connection that provides network time services to SYNC TO NETWORK CLOCK.

Setting and Adjusting System Time:

SET SYSTEM TIME (SYNC TO NETWORK CLOCK)

By default the SYSTEM TIME settings are set to SYNC TO NETWORK CLOCK, which requires a working internet connection to the MAINTENANCE RJ45 port on the BACK PANEL.


SET SYSTEM TIME (SET MANUALLY)

From the SYSTEM SETTINGS menu (A), select SET CLOCK (B), deselect the checkbox for SYNC TO NETWORK CLOCK (C) to disable network clock synchronization, and to allow for setting the Date & Time manually.

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Figure 5.1: Accessing the SET CLOCK functions.

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Animation 5.1: Setting System Time (Manually).

Change any element of the Date or Time by selecting it (e.g., "MM" for "Minutes"), entering a new value, then pressing ENTER. The Date/Time entry (shown in yellow) updates as you make changes, and takes effect once you select "SET CLOCK".

IMPORTANT: AFTER STARTUP THE SYSTEM TIME MAY DISPLAY IN YELLOW (e.g.,  12:45:00  ) TEMPORARILY, THEN CHANGING TO THE COLOR WHITE AFTER SYNCHRONIZING WITH THE NETWORK TIME. IF THE SYSTEM TIME CONTINUES TO DISPLAY IN YELLOW THIS INDICATES THAT THE SYSTEM CLOCK SETTINGS REQUIRE ATTENTION.

WHAT TO DO NEXT:


Select Tubing

The i400 is preloaded with a collection of FACTORY CALIBRATION RECORDS for the most common tubing sizes, and allows for creating an unlimited quantity of USER CALIBRATION RECORDS for your own specific tubing types.

Load Tubing + Select Calibration Record:

LOADING TUBING

The four roller flip-top pumphead loads quickly and is designed for trouble-free continuous use. After verifying the pump rotation is stopped, lift the flip-top to load or unload tubing.

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Animation 6.1: Unloading/Loading tubing into pumphead.

WARNING: KEEP FINGERS AWAY FROM ROTOR WHILE PUMP IS IN OPERATION. STOP PUMP BEFORE LOADING OR UNLOADING TUBING.


SELECT TUBING CALIBRATION RECORD

From the screen-bottom press TASKS button (A), then press TUBING + CAL (B), and use the listbox (C) to select the CALIBRATION RECORD to match the loaded tubing size.

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Figure 6.1: Settings for viewing and selecting a TUBING CALIBRATION RECORD.

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Animation 6.2: Viewing and selecting a CALIBRATION RECORD.

Select any CALIBRATION RECORD to view flow-range details for a specific tubing type, press "DONE" to set the selected record for use with loaded tubing.

WARNING: KEEP FINGERS AWAY FROM ROTOR WHILE PUMP IS IN OPERATION. STOP PUMP BEFORE LOADING OR UNLOADING TUBING.

IMPORTANT: USE ONLY COMPATIBLE TUBING SIZE: 0.5/1.6mm TO 4.8/1.6MM (BORE/WALL).

WHAT TO DO NEXT:


Priming

The i400 has a simple PRIMING interface, allowing for control of priming speed, ROTATION DIRECTION, and making adjustments to TOTALIZERS values.

Setting pumphead Rotation Direction, Priming, and Totalizers:

SET ROTATION DIRECTION

From the screen-top press ROTATION DIRECTION indicator (A) to switch from CW to CCW direction. This setting can only be changed when the pump is idle.

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Figure 7.1: Setting pump ROTATION DIRECTION.


PRIMING & TOTALIZERS

From the screen-bottom press TASKS button (A), then press PRIMING (B), press any droplet icon (C) to start priming, pressing the STOP icon in the center when finished (D). Press CLOSE if finished, or press TOTALIZERS to make any manual adjustment to the totalized values.

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Figure 7.2: Operating the PRIMING function.

The TOTALIZERS pause their recording when PRIMING, and can be manually adjusted to account for additions made during priming. There are two TOTALIZERS shown; CALCULATED TOTAL (mL) and SCALE TOTAL (mL). The calculated total is calculated by tubing and calibration characteristics, while the scale total is based on readings from a connected scale.

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Animation 7.1: PRIMING and adjusting TOTALIZERS.

WHAT TO DO NEXT:


Pump Name

To alleviate confusion when using several pumps in close proximity, you can set a unique PUMP NAME to help distinguish between pumps.

Renaming the pump:

SET PUMP NAME

From the SYSTEM SETTINGS menu (A), select RENAME PUMP (B), use the on-screen keypad (C) to enter a new name for this pump.

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Figure 8.1: Setting PUMP NAME.

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Animation 8.1: Setting the PUMP NAME for display.

WHAT TO DO NEXT:


Manual Operation Mode

The i400 is the only laboratory pump that supports simultaneous mixed-mode operation for ATTENDED (MANUAL MODE), INDUSTRIAL, and REMOTE modes.

Operating the i400 manually:

MANUAL MODE

The MANUAL MODE screen allows you to enter either an RPM value, or a flow rate in mL/min, in a range supported by the calibrated values of the loaded tubing.

NOTE: "INDUSTRIAL" and "REMOTE" modes are covered in a later chapter.

From the screen-bottom press MODE button (A) to toggle from the OVERVIEW screen to the MANUAL MODE screen. Use the MODE button to toggle through each available operational screen.

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Figure 9.1: Switching between operational screens using the MODE button.

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Animation 9.1: Operating in MANUAL MODE.

Changing the mL/min flow rate automatically calculates the precise RPM value, based on tubing calibration records. Adjustments can even be made on the fly while the pumphead is rotating.

WHAT TO DO NEXT:


Dosing

The i400 makes complex dosing schemes easy to configure and run with stored dispensing sequences called "Action Sequences" (referred to as "ASEQ" for short).

Advanced DOSING with Action Sequences (ASEQ):

CREATE SEQUENCE

An ASEQ is composed of (5) parameters:

  • DISPENSE AMOUNT: size of the individual bolus/dose, from 0.1 mL to 1000.0 mL.
  • DISPENSE SPEED: delivery speed of a triggered dosing, in seconds, governed by the loaded tubing size.
  • # OF DELIVERIES: number of doses this ASEQ will deliver over time, from 1x to 9999x.
  • REPEAT INTERVAL: time from when the last dose was triggered, until the next delivery is triggered, from 1 to 86400 seconds (24 hours).
  • RUN TRIGGER: date/time to start the sequence running, with options to RUN NOW, RUN LATER, or STAGE/SAVE the ASEQ for future use.

From the screen-bottom press TASKS button (A), then press CREATE SEQUENCE (B), then follow the on-screen prompts and advance through each screen by pressing the right arrow (C).

NOTE: Upcoming versions of FLUIDLINK/OS enable using multiple independent dispensing sequences. Currently the i400 supports one ASEQ at a time.

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Figure 10.1: Selecting menu items for composing a new ASEQ.

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Animation 10.1: Composing a new ASEQ.

In this example an ASEQ is created for a DISPENSE AMOUNT of 0.5 mL's, at a DISPENSE SPEED 30 seconds, with # OF DELIVERIES of 100x, a REPEAT INTERVAL of 3600 seconds (once an hour), beginning on the 9th of September at 12:30.


RUN STATUS & ASEQ INDICATORS

Understanding each type of RUN STATUS, and using ASEQ EDIT feature:

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    • There are (5) types of RUN STATUS:
    •  ACTIVE  : delivering a scheduled dose.
    •  HALTED  : delivery interrupted by an alarm or operator intervention.
    •  MANUAL  : operator manually pressed RUN button.
    •  NETWORK  : system operated by Industrial Network Interface.
    •  STAGED  : waiting to start the next scheduled dose.
    •  STANDBY  : system is idle with no scheduled dose pending.

    Figure 10.2: Overview of current ASEQ parameters on the OVERVIEW screen.

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    • Pressing the ASEQ EDIT button opens a dialog with the following options:
    • RUN NOW: triggers the saved (idle) ASEQ and changes status from STANDBY to ACTIVE.
    • EDIT TRIGGER: used to cancel a pending trigger, or set a new date/time to trigger a saved ASEQ.
    • STOP RUNNING SEQ: exits a running ASEQ and puts system in STANDBY.

    Figure 10.3: Stop or Modify an ASEQ (trigger) with "ASEQ EDIT" button.

WHAT TO DO NEXT:


Scale Operation

Use a scale* with the i400 to take advantage of ”One Step Calibration” and many other features, including ”Disruption Dampening”, a proprietary disruption dampening algorithm that filters out bad readings caused by accidental bumps.

*Weight scale must support MT-SICS Command Protocol and be equipped with an RS-232 communications interface.

Operating an Integrated Scale:

SCALE OPERATION

From the screen-bottom press TASKS button (A), then press SCALE TASKS (B), and perform TARE and ZERO functions as you would with any standard scale.

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Figure 11.1: Accessing the SCALE operation screen.


LOCK/UNLOCK KEYPAD

From the TASKS: SCALE screen press the MORE... button (A), then select either LOCK KEYPAD or UNLOCK KEYPAD.

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Figure 11.2: Accessing more scale features.

WHAT TO DO NEXT:


Calibration

The i400 can perform an unattended “One-Step Calibration”, executing a series of complex (and time consuming) multi-point calibration processes using scale feedback, and without requiring operator supervision.

NOTE: Calibration is an automated process requiring an integrated scale. Upcoming versions of FLUIDLINK/OS will accommodate "Manual" calibration.

Performing an automated tubing calibration:

SETUP

Supplies and preparation before you press START CAL:

  • Remove any containers from the scale and ZERO the balance to remove any residual TARE values.
  • Place an empty (receiving) bottle with volume enough to contain 1500 mL of media onto the scale.
  • Load tubing and place one end in the supply bottle. Suspend the delivery end of the tubing above the (receiving) bottle on the scale.
  • Ensure the supply bottle contains at least 1500 mL of media.
  • Select the appropriate ROTATION DIRECTION.
  • PRIME the tubing, leaving at least 1450 mL of supply remaining. Do not empty the receiving bottle after priming.

START CALIBRATION

From the screen-bottom press TASKS button (A), then press TUBING + CAL (B), then press CALIBRATE... (C) and press SAVE RECORD AS (D) to begin setting up a calibration routine.

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Figure 12.1: Executing a tubing calibration.


CALIBRATION COMPLETES

The calibration process will run unattended, and may take between 15-45 minutes (A), dependent on the tubing size and its flow rate. Once calibration completes the CLOSE button will display (B), press it to continue. The newly created CALIBRATION RECORD is automatically loaded and displayed (C).

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Figure 12.2: Calibration process ending with newly created record.

Calibration can fail for any number of reasons; running out of supply media, frequent disturbances to the scale, or by being canceled by the operator. In most cases rerunning a calibration yields successful results.

WHAT TO DO NEXT:

  • Continue scrolling to ALARMS.

System Alarms

Alarms will cause your system to be HALTED and require manual intervention by the operator in order to restore operation.

View & Acknowledge Alarms:

VIEW ALARMS

ACTIVE ALARMS can be triggered by different types of events. The i400's screen will flash yellow to alert an operator of an alarm condition.

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Figure 13.1: Screen flashing to indicate an alarm condition.

From the screen-bottom press STATUS button (A), look at the message under ACTIVE ALARMS (B) for alarm details. To acknowledge the alarm press (C). You may be required to leave the alarm active and correct the cause or condition prior to clearing the alarm.

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Figure 13.2: Viewing and clearing an alarm condition.

WHAT TO DO NEXT:


Network Settings

The content for INDUSTRIAL and REMOTE networking settings can be found in this section, or by selecting "NETWORKING" from the menu located in the upper right corner of this screen.

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Figure 14.1: NETWORK SETTINGS menu.

WHAT TO DO NEXT:


Software Update

The i400 Operating System (FLUIDLINK/OS) receives regular service and feature updates. Software updates can be downloaded and installed by the end-user.

CURRENT RELEASE:

Updating the FLUIDLINK/OS:

CHECK INSTALLED VERSION

From the screen-bottom press the STATUS button (A), the currently installed version is listed under the FACTORY INFORMATION (B) heading.

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Figure 15.1: Viewing current FLUIDLINK/OS version.

NOTE: Receive notifications about new updates by joining our mailing list.


BEFORE YOU UPDATE

Software updates are installed via a USB drive (not supplied) which you'll connect to the WEIGHT SCALE port on the BACK PANEL of the i400. After downloading the update ("i400_PACKAGE.zip"), transfer it to the root of a properly formatted USB drive. The drive must be exFAT formatted, and named "i400_UPDATE".


   // USB Drive must be "exFAT" formatted

   Drives/i400_UPDATE/i400_PACKAGE.zip
	


UPDATING FLUIDLINK/OS

From the SYSTEM SETTINGS menu (A), select SHUTDOWN... (B), followed by MORE OPTIONS... (C), press OPTIONS (D) and select SOFTWARE UPDATE from the dialog (E). Follow the system prompts to initiate the update process (F).

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Figure 15.2: Launching the software update program.

WHAT TO DO NEXT:


SYSTEM CARE & MAINTENANCE

Caring for the i400:

During Operation:

Mechanical Maintenance:

  • There are no user-serviceable parts inside the pump, including the pump drive and pump head. The pump should be returned to LAB DYNAMICS or its appointed agent for service.

Cleaning:

  • Keep the pump head clean with mild detergents. Do not immerse or use excessive fluid when cleaning.
  • The pump body and screen can be gently wiped down with 70% Isopropyl Alcohol (IPA).

IMPORTANT: DON'T USE WINDOW CLEANERS, HOUSEHOLD CLEANERS, COMPRESSED AIR, AEROSOL SPRAYS, SOLVENTS, AMMONIA, ABRASIVES, OR CLEANERS CONTAINING HYDROGEN PEROXIDE TO CLEAN THE PUMP. AVOID GETTING MOISTURE IN ANY OPENING, AND DON'T SUBMERGE YOUR PUMP IN ANY CLEANING AGENTS.

WHAT TO DO NEXT:


SAFETY INSTRUCTIONS

Operating the i400 Pump safely:

WARNING: THIS PRODUCT DOES NOT COMPLY WITH THE ATEX DIRECTIVE AND MUST NOT BE USED IN EXPLOSIVE ATMOSPHERES.

WARNING: THESE PRODUCTS ARE NOT DESIGNED FOR USE IN, AND SHOULD NOT BE USED FOR PATIENT-CONNECTED APPLICATIONS.

WARNING: KEEP FINGERS AWAY FROM ROTOR WHILE PUMP IS IN OPERATION. STOP PUMP BEFORE LOADING OR UNLOADING TUBING.

WHAT TO DO NEXT:


APPENDIX

FOR PLACEMENT ONLY:
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WHAT TO DO NEXT: